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HyCyte cell line: HyCyte HEK293 hVPS35 insert GFP in C-terminal

Parental: HEK293

Catalogue No.: CGKI-M2128

Gene: hVPS35

Gene ID: 55737

Passage: 9

Doubling time: 24-48 h

Recommended culture media:

Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCH-G190).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:4-1:8.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Quality test: Bacteria, fungi and mycoplasma negative

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