HyCyte cell line: HyCyte AMO1 hATP1B2 insert EGFP in ATP1B2 gene
Parental: AMO1
Catalogue No.: CGKI-M2146
Gene: hATP1B2
Gene ID: 482
Passage: 9
Doubling time: 72-96 h
Recommended culture media:
RPMI-1640 supplemented with 20 % FBS,100 U/ml Penicilin and 100 µg/ml
Streptomycin (HyCyte,TCH-G575).
Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.
Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).
Quality test: Bacteria, fungi and mycoplasma negative