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HyCyte Cell Line: HyCyte Bewo KO-hABCG2

Parental: Bewo

Catalogue No.: CGKO-M0010

Gene: hABCG2

Gene ID: 9429

Passage: 9

Doubling time: 48-72 h

Clone Type: Homozygote

Recommended culture media:

Ham's F-12K supplemented with 10 % FBS, 2mM Glutamax,1mM Sodium Pyruvate100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCH-G403).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:3.

Freezing media: Ham's F-12K + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: hABCG2 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative