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Human ERAP1 knockout HEK293T cell line
Human ERAP1 knockout HEK293T cell line 
Cat. code: CGKO-M2183
Unit size: 1x10^6
Abbreviation: HEK293T KO-hERAP1
Price:
$2,799.00
General Info
Cell Info
References


HyCyte Cell Line: HyCyte HEK293T KO-hERAP1

Parental: HEK293T

Catalogue No.: CGKO-M2183

Gene: hERAP1

Gene ID: 51752

Passage: 9

Doubling time: 24-48 h

Clone Type: homozygote


Recommended culture media:

Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS, 100 U/mL Penicilin and 100 µg/mL

Streptomycin (HyCyte,TCH-G101).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:4-1:8.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: hERAP1 (-/-)

Quality test: bacteria, fungi and mycoplasma negative


Letko M, et al. Functional assessment of cell entry and receptor usage for SARS-CoV-2 and other lineage B betacoronaviruses. Nat Microbiol 5(4): 562-569, 2020. PubMed: 32094589

Sena-Esteves M, et al. Single-step conversion of cells to retrovirus vector producers with herpes simplex virus-Epstein-Barr virus hybrid amplicons. J. Virol. 73: 10426-10439, 1999. PubMed: 10559361

Pensiero M, et al. Retroviral vectors produced by producer cell lines resistant to lysis by human serum. US Patent 5,952,225 dated Sep 14 1999

Pensiero M, et al. Retroviral vectors produced by producer cell lines resistant to lysis by human serum. US Patent 6,329,199 dated Dec 11 2001   

Pear WS, et al. Production of High-Titer Helper-Free Retroviruses by Transient Transfection. Proc. Natl. Acad. Sci. USA 90: 8392-8396, 1993. PubMed: 7690960

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