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Human ERAP1 knockout 293T cell line
Human ERAP1 knockout 293T cell line 
Cat. code: CGKO-M2183
Unit size: 1x10^6
Abbreviation: 293T KO-hERAP1
Price:
$2,799.00
General Info
Cell Info
References


Modified Gene: hERAP1

Gene ID: 51752

Modification Type: Knockout

Transduction/Transfection: Protein electroporation

Resistance:

Clone Type: homozygote


Cellular Identification: PCR, Sequencing

Genus: homo sapiens

Tissue origin: Embryonic kidney Disease

Cell morphology: Epithelial cells

Characteristics: Adherent

Culture media: DMEM-H+10%FBS+1%P/S

Passage ratio: 1:4-1:8, change the media every 2-3 days.

Passage period: 24-48h

Culture conditions: 95% air + 5% CO2 , 37℃

Freezing conditions: 60% basal medium+30% FBS+10% DMSO, liquid nitrogen storage

Quality test: bacteria, fungi and mycoplasma negative


Letko M, et al. Functional assessment of cell entry and receptor usage for SARS-CoV-2 and other lineage B betacoronaviruses. Nat Microbiol 5(4): 562-569, 2020. PubMed: 32094589

Sena-Esteves M, et al. Single-step conversion of cells to retrovirus vector producers with herpes simplex virus-Epstein-Barr virus hybrid amplicons. J. Virol. 73: 10426-10439, 1999. PubMed: 10559361

Pensiero M, et al. Retroviral vectors produced by producer cell lines resistant to lysis by human serum. US Patent 5,952,225 dated Sep 14 1999

Pensiero M, et al. Retroviral vectors produced by producer cell lines resistant to lysis by human serum. US Patent 6,329,199 dated Dec 11 2001   

Pear WS, et al. Production of High-Titer Helper-Free Retroviruses by Transient Transfection. Proc. Natl. Acad. Sci. USA 90: 8392-8396, 1993. PubMed: 7690960

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