Cellular Identification: PCR, Sequencing
Genus: Cow
Tissue origin: Mammary gland
Cell morphology: Epithelioid
Characteristics: Adherent
Culture media: DMEM-H+10% FBS+1% P/S
Passage ratio: 1:4-1:8, change the media every 2-3 days.
Passage period: 24-36 h
Culture conditions: 95% air + 5% CO2 , 37℃
Freezing conditions: 60% basal medium+30% FBS+10% DMSO, liquid nitrogen storage
Quality test: bacteria, fungi and mycoplasma negative