Accelerate research, empower industry

Modified Gene: Ssb

Gene ID: 100689103

Modification Type: Knockout

Transduction/ Transfection: Protein electroporation

Resistance: Puro

Clone Type: Homozygote

Cellular Identification: PCR, Sequencing

Genus: Hamster

Tissue origin: Ovary

Cell morphology: Epithelioid

Characteristics: Adherent

Culture system: F12K+10%FBS+1%P/S

Passage ratio: 1:3-1:6, change the media every 2-3 days.

Passage period: 48-72 h

Culture conditions: 95% air + 5% CO_{2 ,} 37℃

Freezing conditions: 60% basal medium+30% FBS+10% DMSO, liquid nitrogen storage

Quality test: bacteria, fungi and mycoplasma negative

Puck TT, et al. Genetics of somatic mammalian cells III. Long-term cultivation of euploid cells from human and animal subjects. J. Exp. Med. 108: 945-956, 1958. PubMed: 13598821

Ham RG. Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc. Natl. Acad. Sci. USA 53: 288-293, 1965. PubMed: 14294058

Kao FT, Puck TT. Genetics of somatic mammalian cells, VII. Induction and isolation of nutritional mutants in Chinese hamster cells. Proc. Natl. Acad. Sci. USA 60: 1275-1281, 1968. PubMed: 5244736

Kao FT, Puck TT. Genetics of somatic mammalian cells. IV. Properties of Chinese hamster cell mutants with respect to the requirement for proline. Genetics 55: 513-524, 1967. PubMed: 6068403

Kao FT, et al. Complementation analysis on virus-fused Chinese hamster cells with nutritional markers. Science 164: 312-314, 1969. PubMed: 5813351

Kao F, et al. Genetics of somatic mammalian cells. X. Complementation analysis of glycine-requiring mutants. Proc. Natl. Acad. Sci. USA 64: 1284-1291, 1969. PubMed: 5271752

Mayr GA, Freimuth P. A single locus on human chromosome 21 directs the expression of a receptor for adenovirus type 2 in mouse A9 cells. J. Virol. 71: 412-418, 1997. PubMed: 8985365