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Mouse NPC1 knockout BV-2 cell line
Mouse NPC1 knockout BV-2 cell line 
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Cat.No.： CGKO-M2241 Size： 1x10^6


HyCyte Cell Line: HyCyte BV-2 KO-mNPC1

Parental: BV-2

Catalogue No.: CGKO-M2241

Species: Mouse

Gene: mNPC1

Gene ID: 18145

Passage: 9

Doubling time: 48-72 h

Growth Properties: Semi-adherent

Clone Type: Homozygote

Recommended culture media:

Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCM-G718).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: mNPC1 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative

COA Simple-Set-of-Stationery-Related-Vector-Line-Icons 1.svg


"Woo J.-M., Han D.-H., Park J.-H., Kim S.-J., Kim Y.-S.

In-depth characterization of the secretome of mouse CNS cell lines by LC-MS/MS without prefractionation.

Proteomics 15:3617-3622(2015)

Stansley B., Post J., Hensley K.

A comparative review of cell culture systems for the study of microglial biology in Alzheimer's disease.

J. Neuroinflamm. 9:115-115(2012)

Atwood B.K., Lopez J., Wager-Miller J., Mackie K., Straiker A.

Expression of G protein-coupled receptors and related proteins in HEK293, AtT20, BV2, and N18 cell lines as revealed by microarray analysis.

BMC Genomics 12:14-14(2011)

Crain J.M., Watters J.J.

Estrogen and P2 purinergic receptor systems in microglia: therapeutic targets for neuroprotection.

Open Drug Discov. J. 2:148-167(2010)

Henn A., Lund S., Hedtjarn M., Schrattenholz A., Porzgen P., Leist M.

The suitability of BV2 cells as alternative model system for primary microglia cultures or for animal experiments examining brain inflammation.

ALTEX 26:83-94(2009)"
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