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Human CYP4V2 knockout ARPE-19 cell line
Human CYP4V2 knockout ARPE-19 cell line 
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Cat.No.： CGKO-M2249 Size： 1x10^6

HyCyte Cell Line: HyCyte ARPE-19 KO-hCYP4V2

Parental: ARPE-19

Catalogue No.: CGKO-M2249

Gene: hCYP4V2

Gene ID: 285440

Passage: 9

Doubling time: 48-72 h

Clone Type: Homozygote

Recommended culture media:

Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 (D/F12) supplemented with 10 % FBS,, 100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCH-G127).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: D/F12 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: hCYP4V2 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative

COA Simple-Set-of-Stationery-Related-Vector-Line-Icons 1.svg

"Hazim RA, et al. Rapid differentiation of the human RPE cell line, ARPE-19, induced by nicotinamide. Exp Eye Res 179: 18-24, 2018. PubMed: 30336127

Dunn KC, et al. ARPE-19, A human retinal pigment epithelial cell line with differentiated properties. Exp. Eye Res. 62: 155-169, 1996. PubMed: 8698076

Maidji E, et al. Accessory human cytomegalovirus glycoprotein US9 in the unique short component of the viral genome promotes cell-to-cell transmission of virus in polarized epithelial cells. J. Virol. 70: 8402-8410, 1996. PubMed: 8970961

Holtkamp GM, et al. Polarized secretion of IL-6 and IL-8 by human retinal pigment epithelial cells. Clin. Exp. Immunol. 112: 34-43, 1998. PubMed: 9566787

Finnemann SC, et al. Phagocytosis of rod outer segments by retinal pigment epithelial cells requires alpha(v)beta5 integrin for binding but not for internalization. Proc. Natl. Acad. Sci. USA 94: 12932-12937, 1997. PubMed: 9371778

Handa JT, et al. The advanced glycation endproduct pentosidine induces the expression of PDGF-B in human retinal pigment epithelial cells. Exp. Eye Res. 66: 411-419, 1998. PubMed: 9593635

Dunn KC, et al. Use of the ARPE-19 cell line as a model of RPE polarity: basolateral secretion of FGF5.. Invest. Ophthalmol. Vis. Sci. 39: 2744-2749, 1998. PubMed: 9856785

Tugizov S, et al. An acidic cluster in the cytosolic domain of human cytomegalovirus glycoprotein B is a signal for endocytosis from the plasma membrane. J. Virol. 73: 8677-8688, 1999. PubMed: 10482621

Orten DJ, et al. Analysis of DNA elements that modulate myosin VIIA expression in humans. Hum. Mutat. 14: 354, 1999. PubMed: 10502787

Rajan PD, et al. Expression of the extraneuronal monoamine transporter in RPE and neural retina. Curr. Eye Res. 20: 195-204, 2000. PubMed: 10694895

Janssen JJ, et al. Retinoic acid delays transcription of human retinal pigment neuroepithelium marker genes in ARPE-19 cells. Neuroreport 11: 1571-1579, 2000. PubMed: 10841379

Udono T, et al. Adrenomedullin in cultured human retinal pigment epithelial cells. Invest. Ophthalmol. Vis. Sci. 41: 1962-1970, 2000. PubMed: 10845623

Sparrow JR, et al. The lipofuscin fluorophore A2E mediates blue light-induced damage to retinal pigmented epithelial cells. Invest. Ophthalmol. Vis. Sci. 41: 1981-1989, 2000. PubMed: 10845625"
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