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HyCyte Cell Line: HyCyte MAC-T KO-cwHMOX1

Parental: MAC-T

Catalogue No.: CGKO-M2431

Gene: cwHMOX1

Gene ID: 513221

Passage: 9

Doubling time: 24-36 h

Clone Type: Homozygote

Recommended culture media:

Dulbecco's Modified Eagle Medium ,High Glucose(DMEM)    supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCO-G040).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:4-1:8.

Freezing media: DMEM + 30 % FBS + 10 % DMSO; or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: cwHMOX1 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative