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HyCyte Cell Line: HyCyte SH-SY5Y KO-hUSP16

Parental: SH-SY5Y

Catalogue No.: CGKO-M2461

Species: Human

Gene: hUSP16

Gene ID: 10600

Passage: 9

Doubling time: 48-72 h

Growth Properties: Semi-adherent

Clone Type: Homozygote

Recommended culture media:

Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 (D/F12) supplemented with 15 % FBS, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G325).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:4.

Freezing media: D/F12 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: hUSP16 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative

IF=3.6 Mengzhen Z, Xinwei H, Zeheng T, et al. Integrated machine learning-driven disulfidptosis profiling: CYFIP1 and EMILIN1 as therapeutic nodes in neuroblastoma[J]. Journal of Cancer Research and Clinical Oncology, 2024, 150(3): 109.