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HyCyte Cell Line: HyCyte SiHa KO-hFANCD2

Parental: SiHa

Catalogue No.: CGKO-M2482

Gene: hFANCD2

Gene ID: 2177

Passage: 9

Doubling time: 48-72 h

Clone Type: Homozygote

Recommended culture media:

Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCH-G326).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: DMEM + 30 % FBS + 10 % DMSO; or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: hFANCD2 (-/-)

Quality test: bacteria, fungi and mycoplasma negative

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