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HyCyte Cell Line: HyCyte ARPE-19 KO-hDDIT4

Parental: ARPE-19

Catalogue No.: CGKO-M2585

Gene: hDDIT4

Gene ID: 54541

Passage: 9

Doubling time: 48-72 h

Clone Type: Homozygote

Recommended culture media:

Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 (D/F12) supplemented with 10 % FBS,, 100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCH-G127).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: D/F12 + 30 % FBS+10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: hDDIT4 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative

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