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Human HSP90AA1 knockout SK-HEP-1 cell line
Human HSP90AA1 knockout SK-HEP-1 cell line 
Cat. code: CGKO-M2661
Unit size: 1x10^6
Abbreviation: SK-HEP-1 KO-hHSP90AA1
Price:
$2,999.00
General Info
Cell Info
References


HyCyte Cell Line: HyCyte SK-HEP-1 KO-hHSP90AA1

Parental: SK-HEP-1

Catalogue No.: CGKO-M2661

Gene: hHSP90AA1

Gene ID: 3320

Passage: 9

Doubling time: 24-48 h

Clone Type: Homozygote


Recommended culture media:

Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCM-G329).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: hHSP90AA1 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative



J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080 Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047

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