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HyCyte Cell Line: HyCyte SK-HEP-1 KO-hHSP90AA1

Parental: SK-HEP-1

Catalogue No.: CGKO-M2661

Gene: hHSP90AA1

Gene ID: 3320

Passage: 9

Doubling time: 24-48 h

Clone Type: Homozygote

Recommended culture media:

Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCM-G329).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: hHSP90AA1 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative