Accelerate research, empower industry

CRISPR/Cas9-mediated gene knockout (KO)CRISPR/Cas9-mediated gene knockin (KI)CRISPR/Cas9-mediated gene point-mutationInducible Gene Over-expression (Tet-On)shRNA Knockdown cell serviceCRISPR-KO libraryCRISPRa libraryCRISPRi libraryCRISPRSeek™ library screening serviceCRISPRSeek™ Library PlasmidCRISPRSeek™ Library VirusCRISPRSeek™ Library Cell PoolCRISPRSeek™ Library ProductsHySigen Vector LibraryVB163VB164VB203VB204VB205VB303VB271VB272VB188VB278VB180VB251VB252Custom Vector Construction ServiceCustom Vector Construction Service-1Lentivirus PackagingshRNA AAVAdeno-Associated Virus (AAV) PackagingOver-expression AAVIn-stock virusesVirus PackagingCustomized Peptide SynthesisHuman cell linesAnimal cell linesCell LinesKO cell linesPoint mutation cell linesKI cell linesFluorescence-tag cell linesStable cell linesOff-the-shelf gene-edited cell linesOne-step freezing medium for stem cellsOne-step freezing medium for PBMCsCell freezing mediaOsteogenic differentiation culture mediumAdipogenic differentiation culture mediumChondrogenic differentiation culture mediumStem cell differentiation mediaStem cell culture mediaKnockout Cell LinesKnockin Cell LinesPoint mutation cell lineInducible Gene ExpressionGenome-Wide Libraries at Unbeatable PricesWho we arePartnersDistributorsTechnical PlatformsNews & eventsPublicationsResourcesPrivacy policySupportContact UsSubmitted successfully
插件代码
rLOC102549962 knockout H9c2(2-1) cell line
rLOC102549962 knockout H9c2(2-1) cell line 
Price：
$2,999.00
Cat.No.：
Size：
Quantity：
Order
Product info
Documents
References
Cat.No.： CGKO-M2932 Size： 1x10^6

HyCyte Cell Line: HyCyte H9c2(2-1) KO-rLOC102549962

Parental: H9c2(2-1)

Catalogue No.: CGKO-M2932

Species: Rat

Gene: rLOC102549962

Gene ID: 102549962

Passage: 9

Doubling time: 48-72 h

Growth Properties: Adherent

Clone Type: Homozygote

Recommended culture media:

Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS, 2mM Glutamax,100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCR-G607).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:4.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: rLOC102549962 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative

COA Simple-Set-of-Stationery-Related-Vector-Line-Icons 1.svg

Kimes BW, Brandt BL. Properties of a clonal muscle cell line from rat heart. Exp. Cell Res. 98: 367-381, 1976. PubMed: 943302.

Levy AP, et al. Post-transcriptional regulation of vascular endothelial growth factor by hypoxia. J. Biol. Chem. 271: 2746-2753, 1996. PubMed: 8576250.


IF=3.7 Yan Z, Liu Y, Yang B, et al. Endoplasmic reticulum stress caused by traumatic injury promotes cardiomyocyte apoptosis through acetylation modification of GRP78: GRP78 acetylation modification and cardiomyocyte apoptosis[J]. Acta Biochimica et Biophysica Sinica, 2024, 56(1): 96.

IF=3.3 Mu F, Zhao J, Zhao M, et al. Styrax (Liquidambar orientalis Mill.) promotes mitochondrial function and reduces cardiac damage following myocardial ischemic injury: the role of the AMPK-PGC1α signaling pathway[J]. Journal of Pharmacy and Pharmacology, 2023, 75(12): 1496-1508.

Li W, Peng Y, Liu J, et al. Discovery and Synthesis of Novel Glyrrhizin-Analogs Containing Furanoylpiperazine and the Activity Against Myocardial Injury in Sepsis[J]. Available at SSRN 4821740.
Contact us
Title
Name
*
Country/Region
*
How would you like us to contact you?
*
E-mail
*
Institution
Phone
Where did you learn about HySigen?
Verification code
Change Image
*
Submit

Quote/Order

We will contact you with information about this product. Please let us know how to reach you.
Contact Us


Address: 56 Sugar Creek Blvd Suite 375,
Sugar Land, TX 77478

Email: info@hysigen.com

Telephone: +1 (909) 352-8083 (US)

Accelerate research, empower industry