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Bate-catenin point mutant Hepa1-6 cell line
Bate-catenin point mutant Hepa1-6 cell line 
Cat. code: CGMU-M2137
Unit size: 1x10^6
Abbreviation: Hepa1-6-MU-bate-catenin
Price:
$6,999.00
General Info
Cell Info
References


Modified Gene: bate-catenin_p.Y142E_c.424T>G_c.426T>G

Gene ID: 12387

Modification Type: Point mutation

Transduction/Transfection: Protein electroporation

Clone Type: homozygote


Cellular Identification: PCR, Sequencing

Genus: Mouse

Cell morphology: Epithelial cells

Characteristics: Adherent

Culture media: DMEM-H+10%FBS+1%Glutamax+1% Sodium Pyruvate+1%P/S

Passage ratio: 1:3-1:6, change the media every 2-3 days.

Passage period: 48-72 h

Culture conditions: 95% air + 5% CO2 , 37℃

Freezing conditions: 60% basal medium+30% FBS+10% DMSO, liquid nitrogen storage

Quality test: bacteria, fungi and mycoplasma negative


"Darlington GJ. Liver cell lines. Methods Enzymol. 151: 19-38, 1987. PubMed: 3431441

Darlington GJ, et al. Expression of liver phenotypes in cultured mouse hepatoma cells. J. Natl. Cancer Inst. 64: 809-819, 1980. PubMed: 6102619"

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