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HyCyte cell line: HyCyte NCI-H1299 hHFE_p.C282Y_c.845G>A cell line

Parental: NCI-H1299

Catalogue No.: CGMU-M2157

Gene: hHFE

Gene ID: 3077

Passage: 9

Doubling time: 48-72 h

Recommended culture media:

R

RPMI-1640 supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCH-G269).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).

Quality test: Bacteria, fungi and mycoplasma negative

Giaccone G, et al. Neuromedin B is present in lung cancer cell lines. Cancer Res. 52: 2732s-2736s, 1992. PubMed: 1563005

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Lin DL, Chang C. p53 is a mediator for radiation-repressed human TR2 orphan receptor expression in MCF-7 cells, a new pathway from tumor suppressor to member of the steroid receptor superfamily. J. Biol. Chem. 271: 14649-14652, 1996. PubMed: 8663350

This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates from a lymph node metastasis from a patient who had received prior radiation therapy.