Recommended culture media:
Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 (D/F12) supplemented with 12.5 % FBS,2mM Glutamax, 100 U/ml Penicilin and 100 µg/ml
Streptomycin (HyCyte,TCH-G119).
Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.
Freezing media: D/F12 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).
Quality test: Bacteria, fungi and mycoplasma negative