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HyCyte cell line: HyCyte AC16 hRYR2_p.D179E_c.537T>G cell line

Parental: AC16

Catalogue No.: CGMU-M2170

Gene: hRYR2

Gene ID: 6262

Passage: 9

Doubling time: 48-72 h

Recommended culture media:

Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 (D/F12) supplemented with 12.5 % FBS,2mM Glutamax, 100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCH-G119).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: D/F12 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Quality test: Bacteria, fungi and mycoplasma negative

Novel cell lines derived from adult human ventricular cardiomyocytes.J. Mol.Cell.Cardiol.39:133-147(2005)