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Stable-overexpressing human NLRC5 in MRC-5 cell line
Stable-overexpressing human NLRC5 in MRC-5 cell line 
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Cat.No.： CGSC-M22841 Size： 1x10^6

Cell name: Stable-overexpressing human NLRC5 in MRC-5 cell line

Catalogue No.: CGSC-M22841

Modified Gene: NLRC5

Gene ID: 84166

Modification Type: Overexpression

Method: Piggy-BAC

Transcript: NM_032206.5

Resistance gene: puro

Fluorescent gene: EGFP

Clone Type: Poolcells

Cellular Identification: PCR, Sequencing

Genus: Homo sapiens

Cell morphology: Fibroblasts

Characteristics: Adherent

Culture media: DMEM-H+10%FBS+1%P/S

Passage ratio: 1:3-1:6, change the media every 2-3 days.

Passage period: 48-72h

Culture conditions: 95% air + 5% CO2 , 37℃

Freezing conditions: 60% basal medium+30% FBS+10% DMSO, liquid nitrogen storage

Quality test: bacteria, fungi and mycoplasma negative

COA

Jacobs JP, et al. Characteristics of a human diploid cell designated MRC-5. Nature 227: 168-170, 1970. PubMed: 4316953.

Proc. Symp. Human Diploid Cells. Zagreb, Yugoslavia: Yugoslav Acad. Sci. Arts; 1970.

Holliday R, Tarrant GM. Altered enzymes in ageing human fibroblasts. Nature 238: 26-30, 1972. PubMed: 12635262

Lewis CM, Tarrant GM. Error theory and ageing in human diploid fibroblasts. Nature 239: 316-318, 1972. PubMed: 12635218

Landers JE, et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997. PubMed: 9270029

Kaur A, et al. Cytotoxic T-lymphocyte responses to cytomegalovirus in normal and simian immunodeficiency virus-infected Rhesus macaques. J. Virol. 70: 7725-7733, 1996. PubMed: 8892893

Biological evaluation of medical devices. Part 5: Tests for in vitro cytotoxicity. Sydney, NSW, Australia:Standards Australia;Standards Australia AS ISO 10993.5-2002.

Biological evaluation of medical devices--Part 5: Tests for in vitro cytotoxicity. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 10993-5:1999.

Standard Test Method for Determining the Virus-Eliminating Effectiveness of Liquid Hygienic Handwash and Handrub Agents Using the Fingerpads of Adult Volunteers. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1838-02.

Standard Test Method for Evaluation of Handwashing Formulation for Virus-Eliminating Activity using the Entire Hand. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 2011-99.

Standard Quantitative Disk Carrier Test Method for Determining the Bactericidal, Virucidal, Fungicidal, Mycobactericidal and Sporicidal Activities of Liquid Chemical Germicides. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 2197-02.
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