Cellular Identification: PCR, Sequencing
Genus:Mus musculus
Tissue origin: Colon
Cellular morphology:Fibroblast-like
Characteristics:Adherent
Culture media: RPMI-1640+10%FBS+5.0 ug/mL Puromycin+1%P/S
Passage ratio: 1:3-1:6 , change the media every 2-3 days.
Passage period: 48-72 h
Culture conditions: 95% air + 5% CO2 , 37℃
Freezing conditions: 60% basal medium+30% FBS+10% DMSO, liquid nitrogen storage
Quality test: bacteria, fungi and mycoplasma negative