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Stable-overexpressing human EGFR in Hela cell line
Stable-overexpressing human EGFR in Hela cell line 
Cat. code: CGSC-M23111
Unit size: 1x10^6
Price:
$3,499.00
General Info
Cell Info
References


Modified Gene: hEGFR

Gene ID: 1956

Modification Type: Overexpression

Method: Piggy-BAC

Transcript: NM_005228.5

Resistance gene: puro

Fluorescent gene:

Clone Type: Poolcells


Cellular Identification: PCR, Sequencing

Genus: Homo sapiens

Cell morphology: Epithelial cells

Characteristics: Adherent

Culture media: MEM+10%FBS+1%P/S

Passage ratio: 1:2-1:6, change the media every 2-3 days.

Passage period: 48-72 h

Culture conditions: 95% air + 5% CO2 , 37℃

Freezing conditions: 60% basal medium+30% FBS+10% DMSO, liquid nitrogen storage

Quality test: bacteria, fungi and mycoplasma negative


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Baldi A, et al. Genomic structure of the human retinoblastoma-related Rb2/p130 gene. Proc. Natl. Acad. Sci. USA 93: 4629-4632, 1996. PubMed: 8643454

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Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217

Scheffner M, et al. The state of the p53 and retinoblastoma genes in human cervical carcinoma cell lines. Proc. Natl. Acad. Sci. USA 88: 5523-5527, 1991. PubMed: 1648218

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