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Stable-overexpressing human HLF in HCT116 cell line
Stable-overexpressing human HLF in HCT116 cell line 
Cat. code: CGSC-M25781
Unit size: 1x10^6
Price:
$3,499.00
General Info
Cell Info
References


Modified Gene: hHLF

Gene ID: 3131

Modification Type: Overexpression

Method: LV

Transcript: NM_002126.5

Resistance gene: Puro

Fluorescent gene: EGFP

Clone Type: Poolcells


Cellular Identification: PCR, Sequencing

GenusHomo sapiens

Tissue originColon

Cellular morphologyEpithelioid

CharacteristicsAdherent

Culture media: McCoy's 5A+10%FBS+1.0 ug/mL Puromycin+1%P/S

Passage ratio: 1:3-1:8 , change the media every 2-3 days.

Passage period: 48-72 h

Culture conditions: 95% air + 5% CO2 , 37℃

Freezing conditions: 60% basal medium+30% FBS+10% DMSO, liquid nitrogen storage

Quality test: bacteria, fungi and mycoplasma negative


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Brattain MG, et al. Enhancement of growth of human colon tumor cell lines by feeder layers of murine fibroblasts. J. Natl. Cancer Inst. 69: 767-771, 1982. PubMed: 6956756

Bender CM, et al. Inhibition of DNA methylation by 5-Aza-2'-deoxycytidine suppresses the growth of human tumor cell lines. Cancer Res. 58: 95-101, 1998. PubMed: 9426064

Landers JE, et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997. PubMed: 9270029

Kutchera W, et al. Protaglandin H synthase 2 is expressed abnormally in human colon cancer: evidence for a transcriptional effect. Proc. Natl. Acad. Sci. USA 93: 4816-4820, 1996. PubMed: 8643486

Wang R, et al. Cellular adherence elicits ligand-independent activation of the Met cell-surface receptor. Proc. Natl. Acad. Sci. USA 93: 8425-8430, 1996. PubMed: 8710887"

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