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Modified Gene: hANXA1

Gene ID: 301

Modification Type: Overexpression

Method: Piggy-BAC

Transcript: NM_000700.2

Resistance gene: Puro

Fluorescent gene: EGFP

Clone Type: Poolcells

Cellular Identification: PCR, Sequencing

Genus：Homo sapiens

Tissue origin：Kidney

Cellular morphology：Epithelioid

Characteristics：Adherent

Culture media: McCoy's 5A＋10% FBS＋1% P/S

Passage ratio: 1:2-1:4 , change the media every 2-3 days.

Passage period: 24-36 h

Culture conditions: 95% air + 5% CO_{2 ,} 37℃

Freezing conditions: 60% basal medium+30% FBS+10% DMSO, liquid nitrogen storage

Quality test: bacteria, fungi and mycoplasma negative