Cellular Identification: PCR, Sequencing
Genus: homo sapiens
Tissue origin: Brain
Cell morphology: Epithelial cells
Characteristics: Semi-adherent
Culture media: MEM/F12+15% FBS+1% P/S
Passage ratio: 1:3-1:4, change the media every 2-3 days.
Passage period: 48-72 h
Culture conditions: 95% air + 5% CO2 , 37℃
Freezing conditions: 60% basal medium+30% FBS+10% DMSO, liquid nitrogen storage
Quality test: bacteria, fungi and mycoplasma negative