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Stable-overexpressing human GRAMD1C in Huh7 cell line
Stable-overexpressing human GRAMD1C in Huh7 cell line 
Cat. code: CGSC-M26261
Unit size: 1x10^6
Price:
$3,499.00
General Info
Cell Info
References


Modified Gene: hGRAMD1C

Gene ID: 54762

Modification Type: Overexpression

Method: Piggy-BAC

Transcript: NM_017577.5

Resistance gene: Puro

Fluorescent gene: EGFP

Clone Type: Poolcells


Cellular Identification: PCR, Sequencing

Genus: Homo sapiens

Tissue originLiver

Cell morphology: Epithelial cells

Characteristics: Adherent

Culture media: DMEM-H+10%FBS+1%Glutamax+1% Sodium Pyruvate+1%P/S

Passage ratio: 1:2-1:3, change the media every 2-3 days.

Passage period: 48-72h

Culture conditions: 95% air + 5% CO2 , 37℃

Freezing conditions: 60% basal medium+30% FBS+10% DMSO, liquid nitrogen storage

Quality test: bacteria, fungi and mycoplasma negative


Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A.

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Cancer Res. 79:1263-1273(2019)

Kasai F., Hirayama N., Ozawa M., Satoh M., Kohara A.

HuH-7 reference genome profile: complex karyotype composed of massive loss of heterozygosity.

Hum. Cell 31:261-267(2018)

Shi J., Wang X., Lyu L., Jiang H., Zhu H.-J.

Comparison of protein expression between human livers and the hepatic cell lines HepG2, Hep3B, and Huh7 using SWATH and MRM-HR proteomics: Focusing on drug-metabolizing enzymes.

Drug Metab. Pharmacokinet. 33:133-140(2018)

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