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Stable-overexpressing human CACNA1H in HEK-293 cell line
Stable-overexpressing human CACNA1H in HEK-293 cell line 
Cat. code: CGSC-M26511
Unit size: 1x10^6
Price:
$3,499.00
General Info
Cell Info
References


Modified Gene: hCACNA1H

Gene ID: 8912

Modification Type: Overexpression

Method: Piggy-BAC

Transcript: NM_021098.3

Resistance gene: puro

Fluorescent gene:

Clone Type: Poolcells/MixClone


Cellular Identification: PCR, Sequencing

Genus: homo sapiens

Tissue origin: Embryonic kidney Disease

Cell morphology: Epithelial cells

Characteristics: Adherent

Culture media: DMEM-H+10%FBS+1%P/S

Passage ratio: 1:4-1:8, change the media every 2-3 days.

Passage period: 24-48 h

Culture conditions: 95% air + 5% CO2 , 37℃

Freezing conditions: 60% basal medium+30% FBS+10% DMSO, liquid nitrogen storage

Quality test: bacteria, fungi and mycoplasma negative


"Xie QW, et al. Complementation analysis of mutants of nitric oxide synthase reveals that the active site requires two hemes. Proc. Natl. Acad. Sci. USA 93: 4891-4896, 1996. PubMed: 8643499

Da Costa LT, et al. Converting cancer genes into killer genes. Proc. Natl. Acad. Sci. USA 93: 4192-4196, 1996. PubMed: 8633039

Graham FL, et al. Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J. Gen. Virol. 36: 59-72, 1977. PubMed: 886304

Graham FL, et al. Defective transforming capacity of adenovirus type 5 host-range mutants. Virology 86: 10-21, 1978. PubMed: 664220

Harrison T, et al. Host-range mutants of adenovirus type 5 defective for growth in HeLa cells. Virology 77: 319-329, 1977. PubMed: 841862

Bodary SC, McLean JW. The integrin beta 1 subunit associates with the vitronectin receptor alpha v subunit to form a novel vitronectin receptor in a human embryonic kidney cell line. J. Biol. Chem. 265: 5938-5941, 1990. PubMed: 1690718

Goodrum FD, Ornelles DA. The early region 1B 55-kilodalton oncoprotein of adenovirus relieves growth restrictions imposed on viral replication by the cell cycle. J. Virol. 71: 548-561, 1997. PubMed: 8985383"

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