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Modified Gene: ggaOVAL

Gene ID: 396058

Modification Type: Overexpression

Method: Piggy-BAC

Transcript: NM_205152.3   

Resistance gene: Puro

Fluorescent gene: Luciferase

Clone Type: Poolcells

Cellular Identification: PCR, Sequencing

Genus: Mus musculus

Tissue origin: Pancreas

Cell morphology: Epithelial cells

Characteristics: Adherent

Culture media: RPMI-1640＋10% FBS+1%P/S

Passage ratio: 1:2-1:4, change the media every 2-3 days.

Passage period: 36-48 h

Culture conditions: 95% air + 5% CO₂ , 37℃

Freezing conditions: 60% basal medium+30% FBS+10% DMSO, liquid nitrogen storage

Quality test: bacteria, fungi and mycoplasma negative