Accelerate research, empower industry

Product Name：Mesenchymal Stem Cell Complete Medium

Catalog No.： GUMD-D101

Size：200 mL

Product Introduction

Under the stimulation of the induction medium, mesenchymal stem cells (MSCs) gradually differentiate toward osteogenic lineage, exhibiting a pronounced calciumsecretion response. This leads to the formation of calcium salt crystals or mineralized nodules, which can be stained with Alizarin Red. This product is designed for osteogenic induction of adipose-derived mesenchymal stem cells and can significantly enhance the induction efficiency.

Product Features

· Stable product performance that markedly improves the osteogenic induction efficiency of multi-derived mesenchymal stem cells.

· Includes staining solution for added convenience.

· Ready-to-use format – can be used immediately after opening, saving time and effort.

NOTE: The level of osteogenic differentiation of mesenchymal stem cells may vary depending on factors such as cell type, donor source, culture conditions, passage number, cell state, and duration of differentiation.

Example: Osteogenic Induction Procedure

(The following steps are for reference only. Please refer to the instruction manual for details.)

1.Seeding Mesenchymal Stem Cells: Use cells in the logarithmic growth phase and seed them at a density of 2×10⁴ cells/cm² onto precoated culture vessels. Incubate at 37°C, 5% CO₂ until the confluency reaches 60-70%. Discard the supernatant and add osteogenic induction differentiation medium.

2.Cell Differentiation Induction: Replace the osteogenic induction medium every 2–3 days. Culture at 37°C, 5% CO₂ for approximately 14-21 days, while observing changes in cell morphology. Determine the appropriate time to terminate the induction based on the appearance of calcium salt crystals and mineralized nodules, then proceed to staining for identification.

3.Cell Fixation: Aspirate the medium and rinse once with an appropriate amount of 1×PBS. Discard the PBS, then cover the bottom of the culture vessel with an appropriate amount of 4% neutral formaldehyde solution. Fix at room temperature for 30–60 minutes. Discard the fixative and rinse twice with 1×PBS.

4. Alizarin Red Staining: Add an appropriate amount of Alizarin Red staining solution and stain for 3–5 minutes. Aspirate the staining solution, rinse twice with 1×PBS, and add a suitable amount of 1×PBS to prevent the cells from drying out.

5.Induction Evaluation: Observe the osteogenic staining effect under a microscope, capture images, and evaluate the induction results. Successful induction is indicated by the presence of mineralized nodules that bind with Alizarin Red, appearing red or orangered in color.