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Product Name：Luciferase Reporter A-375 Cell Line

HyCyte cell line：HyCyte A-375-Luc

Catalog No.：TCH-C114L

Construction method：Lentivirus

Selection Marker：Puromycin

Parental：A-375

Species：Human

Morphology：Epithelioid

Growth properties：Adherent

Culture Medium：Dulbecco's Modified Eagle Medium ,High Glucose (DMEM)    supplemented with 10 % FBS,2.0 ug/mL Puromycin, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G114L）

Passage：1:3-1:4

Doubling time：24-48 h

Subculture：Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:4.

Freezing media：DMEM+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).