Parental:A-375
Species:Human
Morphology:Epithelioid
Growth properties:Adherent
Culture Medium:Dulbecco's Modified Eagle Medium ,High Glucose (DMEM) supplemented with 10 % FBS,2.0 ug/mL Puromycin, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G114L)
Passage:1:3-1:4
Doubling time:24-48 h
Subculture:Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:4.
Freezing media:DMEM+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).