Parental:A-431
Species:Human
Morphology:Epithelioid
Growth properties:adherent
Culture Medium: DMEM-H+10%FBS+1% Glutamax+1% Sodium Pyruvate+2.0 ug/mL Puromycin+1%P/S
Passage: 1:3-1:6
Doubling time:36-48 h
Subculture:Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:4.
Freezing media:DMEM+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).