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Luciferase Reporter A-549 Cell Line(A-549-Luc)
Luciferase Reporter A-549 Cell Line(A-549-Luc) 
Cat. code： TCH-C116L
Unit size： 1x10^6
Abbreviation： HyCyte A-549-Luc
Price：
$1,260.00
General Info
Cell Info
References


Product Name：Luciferase Reporter A-549 Cell Line

HyCyte cell line：HyCyte A-549-Luc

Cat. code：TCH-C116L

Construction method：PiggyBac

Selection Marker：Puromycin


Parental：A-549

Species：Human

Morphology：Epithelioid

Growth properties：Adherent

Culture Medium：Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 (D/F12) supplemented with 10 % FBS,2.0 ug/mL Puromycin, 100 U/ml Penicilin and 100 µg/mlStreptomycin (HyCyte,TCH-G116L).

Passage：1:3-1:6

Doubling time：48-72 h

Subculture：Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media：D/F12+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).


Letko M, et al. Functional assessment of cell entry and receptor usage for SARS-CoV-2 and other lineage B betacoronaviruses. Nat Microbiol 5(4): 562-569, 2020. PubMed: 32094589

Giard DJ, et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758

Mayr GA, Freimuth P. A single locus on human chromosome 21 directs the expression of a receptor for adenovirus type 2 in mouse A9 cells. J. Virol. 71: 412-418, 1997. PubMed: 8985365

Goodrum FD, Ornelles DA. The early region 1B 55-kilodalton oncoprotein of adenovirus relieves growth restrictions imposed on viral replication by the cell cycle. J. Virol. 71: 548-561, 1997. PubMed: 8985383

St. Geme JW, et al. Characterization of the genetic locus encoding Haemophilus influenzae type b surface fibrils. J. Bacteriol. 178: 6281-6287, 1996. PubMed: 8892830

Horikami SM, et al. The Sendai virus V protein interacts with the NP protein to regulate viral genome RNA replication. Virology 222: 383-390, 1996. PubMed: 8806522

Huang S, et al. Adenovirus interaction with distinct integrins mediates separate events in cell entry and gene delivery to hematopoietic cells. J. Virol. 70: 4502-4508, 1996. PubMed: 8676475"
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