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Luciferase labelled human renal cell adenocarcinoma cells(ACHN-Luc)
Luciferase labelled human renal cell adenocarcinoma cells(ACHN-Luc) 
Cat. code: TCH-C122L
Unit size: 1x10^6
Abbreviation: ACHN-Luc
Price:
$1,260.00
General Info
Cell Info
References


Cell nameLuciferase labelled human renal cell adenocarcinoma cells

AbbreviationACHN-Luc

Cat. codeTCH-C122L

Transduction:LV

Resistance:Puro


GenusHomo sapiens

Tissue originKidney

Cellular morphologyEpithelioid

CharacteristicsAdherent

Culture media: DMEM-H+10% FBS+1%Glutamax+1% Sodium Pyruvate+1%NEAA+2.0 ug/mL Puromycin+1%P/S

Passage ratio: 1:2-1:3 , change the media every 2-3 days.

Passage period: 24-48 h

Culture conditions: 95% air + 5% CO2 , 37℃

Freezing conditions: 60% basal medium+30% FBS+10% DMSO, liquid nitrogen storage

Quality test: bacteria, fungi and mycoplasma negative


Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells The ACHN cell line was initiated in November, 1979 from the malignant pleural effusion of a 22-year-old Caucasian male with widely metastatic renal adenocarcinoma (autopsy confirmed). Cells were seeded directly to culture flasks in Eagle's MEM with 10% FBS, then maintained and passaged 150 days in flasks. Cells were then inoculated subcutaneously into nude mice. After 4 weeks, palpable, locally invasive tumors were noted. Both the original cells (ACHN) and those recovered from nude mouse tumors were growth-inhibited by human interferons. Kochevar J. Blockage of autonomous growth of ACHN cells by anti-renal cell carcinoma monoclonal antibody 5F4. Cancer Res. 20: 2968-2972, 1990. PubMed: 2334900

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