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Luciferase Reporter ACHN Cell Line(ACHN-Luc)
Luciferase Reporter ACHN Cell Line(ACHN-Luc) 
Cat. code： TCH-C122L
Unit size： 1x10^6
Abbreviation： HyCyte ACHN-Luc
Price：
$1,260.00
General Info
Cell Info
References


Product Name：Luciferase Reporter ACHN Cell Line

HyCyte cell line：HyCyte ACHN-Luc

Catalog No.：TCH-C122L

Construction method：Lentivirus

Selection Marker：Puromycin


Parental：ACHN

Species：Human

Morphology：Epithelioid

Growth properties：Adherent

Culture Medium：Dulbecco's Modified Eagle Medium ,High Glucose (DMEM)    supplemented with 10 % FBS,2.0 ug/mL Puromycin, 2mM Glutamax, 1mM Sodium Pyruvate,1%NEAA,100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G122L）

Passage：1:2-1:3

Doubling time：24-48 h

Subculture：Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:3.

Freezing media：DMEM+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).


Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells The ACHN cell line was initiated in November, 1979 from the malignant pleural effusion of a 22-year-old Caucasian male with widely metastatic renal adenocarcinoma (autopsy confirmed). Cells were seeded directly to culture flasks in Eagle's MEM with 10% FBS, then maintained and passaged 150 days in flasks. Cells were then inoculated subcutaneously into nude mice. After 4 weeks, palpable, locally invasive tumors were noted. Both the original cells (ACHN) and those recovered from nude mouse tumors were growth-inhibited by human interferons. Kochevar J. Blockage of autonomous growth of ACHN cells by anti-renal cell carcinoma monoclonal antibody 5F4. Cancer Res. 20: 2968-2972, 1990. PubMed: 2334900
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