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Luciferase Reporter BxPC-3 Cell Line(BxPC-3-Luc)
Luciferase Reporter BxPC-3 Cell Line(BxPC-3-Luc) 
Cat. code: TCH-C142L
Unit size: 1x10^6
Abbreviation: HyCyte BxPC-3-Luc
Price:
$1,260.00
General Info
Cell Info
References


Product NameLuciferase Reporter BxPC-3 Cell Line

HyCyte cell lineHyCyte BxPC-3-Luc

Catalog No.TCH-C142L

Construction method:Lentivirus

Selection Marker:Puromycin


ParentalBxPC-3

SpeciesHuman

MorphologyEpithelioid

Growth propertiesAdherent

Culture Medium:RPMI-1640 supplemented with 10 % FBS,2.0ug/mL Puromycin,   100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G142L).

Passage:1:3-1:4

Doubling time:48-60 h

Subculture:Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:4.

Freezing media:RPMI-1640+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).


Loor R , et al. Use of pancreas-specific antigen in immunodiagnosis of pancreatic cancer. Clin. Lab. Med. 2: 567-578, 1982. PubMed: 6754226 Lan MS , et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903 Chambers JA , Harris A . Expression of the cystic fibrosis gene and the major pancreatic mucin gene, MUC1, in human ductal epithelial cells. J. Cell Sci. 105: 417-422, 1993. PubMed: 7691840 Tan MH , et al. Characterization of a new primary human pancreatic tumor line. Cancer Invest. 4: 15-23, 1986. PubMed: 3754176

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