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Luciferase Reporter COLO 205 Cell Line(COLO 205-Luc)
Luciferase Reporter COLO 205 Cell Line(COLO 205-Luc) 
Cat. code： TCH-C161L
Unit size： 1x10^6
Abbreviation： HyCyte COLO 205-Luc
Price：
$1,260.00
General Info
Cell Info
References


Product Name：Luciferase Reporter COLO 205 Cell Line

HyCyte cell line：HyCyte COLO 205-Luc

Catalog No.：TCH-C161L

Construction method：Lentivirus

Selection Marker：Puromycin


Parental：COLO 205

Species：Human

Morphology：Epithelioid

Growth properties：Attached or floating

Culture Medium：RPMI-1640 supplemented with 10 % FBS,2.0 ug/mL Puromycin, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G161L).

Passage：5×10^5-1×10^6cells/mL

Doubling time：24-48 h

Subculture：Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media：RPMI-1640+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).


Semple TU , et al. Tumor and lymphoid cell lines from a patient with carcinoma of the colon for a cytotoxicity model. Cancer Res. 38: 1345-1355, 1978. PubMed: 565251 Gastl GA , et al. Interleukin-10 production by human carcinoma cell lines and its relationship to interleukin-6 expression. Int. J. Cancer 55: 96-101, 1993. PubMed: 8344757 Trainer DL , et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874 Bjork P , et al. Isolation, partial characterization, and molecular cloning of a human colon adenocarcinoma cell-surface glycoprotein recognized by the C215 mouse monoclonal antibody. J. Biol. Chem. 268: 24232-24241, 1993. PubMed: 7693697
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