Accelerate research, empower industry

Luciferase Reporter DLD-1 Cell Line(DLD-1-Luc)
Luciferase Reporter DLD-1 Cell Line(DLD-1-Luc) 
Cat. code： TCH-C169L
Unit size： 1x10^6
Abbreviation： HyCyte DLD-1-Luc
Price：
$1,260.00
General Info
Cell Info
References


Product Name：Luciferase Reporter DLD-1 Cell Line

HyCyte cell line：HyCyte DLD-1-Luc

Catalog No.：TCH-C169L

Construction method：Lentivirus

Selection Marker：Puromycin


Parental：DLD-1

Species：Human

Morphology：Epithelioid

Growth properties：Adherent

Culture Medium：RPMI-1640 supplemented with 10 % FBS,6.0 ug/mL Puromycin, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G169L).

Passage： 1:3-1:4

Doubling time：48-72 h

Subculture：Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:4.

Freezing media： RPMI-1640+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).


Chen TR, et al. Intercellular karyotypic similarity in near-diploid cell lines of human tumor origins. Cancer Genet. Cytogenet. 10: 351-362, 1983. PubMed: 6652615

Chen TR, et al. DLD-1 and HCT-15 cell lines derived separately from colorectal carcinomas have totally different chromosome changes but the same genetic origin. Cancer Genet. Cytogenet. 81: 103-108, 1995. PubMed: 7621404

Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874

Dexter DL, et al. N,N-dimethylformamide-induced alteration of cell culture characteristics and loss of tumorigenicity in cultured human colon carcinoma cells. Cancer Res. 39: 1020-1025, 1979. PubMed: 427742

Rodrigues NR, et al. p53 mutations in colorectal cancer. Proc. Natl. Acad. Sci. USA 87: 7555-7559, 1990. PubMed: 1699228

Keesee SK, et al. Nuclear matrix proteins in human colon cancer. Proc. Natl. Acad. Sci. USA 91: 1913-1916, 1994. PubMed: 8127905

Kutchera W, et al. Protaglandin H synthase 2 is expressed abnormally in human colon cancer: evidence for a transcriptional effect. Proc. Natl. Acad. Sci. USA 93: 4816-4820, 1996. PubMed: 8643486

Vermeulen SJ, et al. Did the four human cancer cell lines DLD-1, HCT-15, HCT-8, and HRT-18 originate from one and the same patient?. Cancer Genet. Cytogenet. 107: 76-79, 1998. PubMed: 9809040
Contact us
Title
Name
*
Country/Region
*
How would you like us to contact you?
*
E-mail
*
Institution
Phone
Verification code
Change Image
*
Submit

Quote/Order

We will contact you with information about this product. Please let us know how to reach you.
Contact Us


Address: 56 Sugar Creek Blvd Suite 375, Sugar Land, TX 77478

Email: info@hysigen.com

Telephone: 628-777-8169 (US)

Accelerate research, empower industry