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Luciferase Reporter DU145 Cell Line(DU145-Luc)
Luciferase Reporter DU145 Cell Line(DU145-Luc) 
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Cat.No.： TCH-C170L Size： 1x10^6

Product Name：Luciferase Reporter DU145 Cell Line

HyCyte cell line：HyCyte DU145-Luc

Catalog No.：TCH-C170L

Construction method：Lentivirus

Selection Marker：Puromycin

Parental：DU145

Species：Human

Morphology：Epithelial-like

Growth properties：adherent

Culture Medium: Dulbecco's Modified Eagle Medium ,High Glucose (DMEM)    supplemented with 10 % FBS,8.0 ug/mL Puromycin, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G170L）

Passage : 1:3-1:6

Doubling time：48-72 h

Subculture : Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media : DMEM+ 30 % FBS + 10 % DMSO; or One Step Freezing Medium (HyCyte, GUCP-R201).


Papsidero LD, et al. Prostate antigen: a marker for human prostate epithelial cells. J. Natl. Cancer Inst. 66: 37-42, 1981. PubMed: 6935463

Stone KR, et al. Isolation of a human prostate carcinoma cell line (DU 145). Int. J. Cancer 21: 274-281, 1978. PubMed: 631930

Mickey DD, et al. Heterotransplantation of a human prostatic adenocarcinoma cell line in nude mice. Cancer Res. 37: 4049-4058, 1977. PubMed: 908039

Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212

Hu SX, et al. Development of an adenovirus vector with tetracycline-regulatable human tumor necrosis factor alpha gene expression. Cancer Res. 57: 3339-3343, 1997. PubMed: 9269991

Sheng S, et al. Maspin acts at the cell membrane to inhibit invasion and motility of mammary and prostatic cancer cells. Proc. Natl. Acad. Sci. USA 93: 11669-11674, 1996. PubMed: 8876194

Carter RE, et al. Prostate-specific membrane antigen is a hydrolase with substrate and pharmacologic characteristics of a neuropeptidase. Proc. Natl. Acad. Sci. USA 93: 749-753, 1996. PubMed: 8570628
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