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Product Name：Luciferase Reporter DU145 Cell Line

HyCyte cell line：HyCyte DU145-Luc

Catalog No.：TCH-C170L

Construction method：Lentivirus

Selection Marker：Puromycin

Parental：DU145

Species：Human

Morphology：Epithelial-like

Growth properties：adherent

Culture Medium: Dulbecco's Modified Eagle Medium ,High Glucose (DMEM)    supplemented with 10 % FBS,8.0 ug/mL Puromycin, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G170L）

Passage : 1:3-1:6

Doubling time：48-72 h

Subculture : Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media : DMEM+ 30 % FBS + 10 % DMSO; or One Step Freezing Medium (HyCyte, GUCP-R201).

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