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Luciferase Reporter HCC827 Cell Line(HCC827-Luc)
Luciferase Reporter HCC827 Cell Line(HCC827-Luc) 
Cat. code: TCH-C182L
Unit size: 1x10^6
Abbreviation: HyCyte HCC827-Luc
Price:
$1,260.00
General Info
Cell Info
References


Product NameLuciferase Reporter HCC827 Cell Line

HyCyte cell lineHyCyte HCC827-Luc

Catalog No.TCH-C182L

Construction method:Lentivirus

Selection Marker:Puromycin


ParentalHCC827

SpeciesHuman

MorphologyEpithelioid

Growth propertiesAdherent

Culture Medium:RPMI-1640 supplemented with 10 % FBS,2.0ug/mL Puromycin,   100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G182L).

Passage:1:3-1:4

Doubling time:24-48 h

Subculture:Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:4.

Freezing media:RPMI-1640+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).


Girard L , et al. Genome-wide allelotyping of lung cancer identifies new regions of allelic loss, differences between small cell lung cancer and non-small cell lung cancer, and loci clustering. Cancer Res. 60: 4894-4906, 2000. PubMed: 10987304 Burbee DG , et al. Epigenetic inactivation of RASSF1A in lung and breast cancers and malignant phenotype suppression. J. Natl. Cancer Inst. 93: 691-699, 2001. PubMed: 11333291 Toyooka S , et al. Differential expression of FEZ1/LZTS1 gene in lung cancers and their cell cultures. Clin. Cancer Res. 8: PubMed: 12114433 Virmani A , et al. Aberrant methylation of the cyclin D2 promoter in primary small cell, nonsmall cell lung and breast cancers. Int. J. Cancer 107: 341-345, 2003. PubMed: 14506731 Liu CX , et al. LRP-DIT, a putative endocytic receptor gene, is frequently inactivated in non-small cell lung cancer cell lines. Cancer Res. 60: 1961-1967, 2000. PubMed: 10766186 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Centers for Disease Control (1993), Biosafety in Microbiological and Biomedical Laboratories Human Health Service Publication No. (CDC) 93-8395. U.S. Dept. of Health and Human Services; 3rd Edition U.S. Government Printing Office Washington D.C.

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