Accelerate research, empower industry

Luciferase Reporter HCT116 Cell Line(HCT116-Luc)
Luciferase Reporter HCT116 Cell Line(HCT116-Luc) 
Cat. code: TCH-C185L
Unit size: 1x10^6
Abbreviation: HyCyte HCT116-Luc
Price:
$1,260.00
General Info
Cell Info
References


Product NameLuciferase Reporter HCT116 Cell Line

HyCyte cell lineHyCyte HCT116-Luc

Catalog No.TCH-C185L

Construction method:Lentivirus

Selection Marker:Puromycin


ParentalHCT116

SpeciesHuman

MorphologyEpithelioid

Growth propertiesAdherent

Culture Medium: McCoy's 5A Medium supplemented with 10 % FBS, 1.0ug/mL Puromycin,100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G185L).

Passage:1:3-1:8

Doubling time:48-72 h

Subculture:Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:8.

Freezing media:McCoy's 5A+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).


"Park SM, et al. The miR-200 family determines the epithelial phenotype of cancer cells by targeting the E-cadherin repressors ZEB1 and ZEB2. Genes Dev 22(7): 894-907, 2008. PubMed: 18381893

Schroy PC, et al. Detection of p21ras mutations in colorectal adenomas and carcinomas by enzyme-linked immunosorbent assay. Cancer 76: 201-209, 1995. PubMed: 8625092

Brattain MG, et al. Heterogeneity of malignant cells from a human colonic carcinoma. Cancer Res. 41: 1751-1756, 1981. PubMed: 7214343

Sun L, et al. Autocrine transforming growth factor-beta 1 and beta 2 expression is increased by cell crowding and quiescence in colon carcinoma cells. Exp. Cell Res. 214: 215-224, 1994. PubMed: 8082724

Santoro IM, Groden J. Alternative splicing of the APC gene and its association with terminal differentiation. Cancer Res. 57: 488-494, 1997. PubMed: 9012479

Brattain MG, et al. Enhancement of growth of human colon tumor cell lines by feeder layers of murine fibroblasts. J. Natl. Cancer Inst. 69: 767-771, 1982. PubMed: 6956756

Bender CM, et al. Inhibition of DNA methylation by 5-Aza-2'-deoxycytidine suppresses the growth of human tumor cell lines. Cancer Res. 58: 95-101, 1998. PubMed: 9426064

Landers JE, et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997. PubMed: 9270029

Kutchera W, et al. Protaglandin H synthase 2 is expressed abnormally in human colon cancer: evidence for a transcriptional effect. Proc. Natl. Acad. Sci. USA 93: 4816-4820, 1996. PubMed: 8643486

Wang R, et al. Cellular adherence elicits ligand-independent activation of the Met cell-surface receptor. Proc. Natl. Acad. Sci. USA 93: 8425-8430, 1996. PubMed: 8710887"

Contact us
Title
Name
*
Country/Region
*
How would you like us to contact you?
*
E-mail
*
Institution
Phone
Verification code
 Change Image
*
Submit

Quote/Order

We will contact you with information about this product. Please let us know how to reach you.

Contact Us


Address: 56 Sugar Creek Blvd Suite 375, Sugar Land, TX 77478

Email: info@hysigen.com

Telephone: 628-777-8169 (US)

Accelerate research, empower industry