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Luciferase Reporter HCT 15 Cell Line(HCT 15-Luc)
Luciferase Reporter HCT 15 Cell Line(HCT 15-Luc) 
Cat. code： TCH-C186L
Unit size： 1x10^6
Abbreviation： HyCyte HCT 15-Luc
Price：
$1,260.00
General Info
Cell Info
References


Product Name：Luciferase Reporter HCT 15 Cell Line

HyCyte cell line：HyCyte HCT 15-Luc

Catalog No.：TCH-C186L

Construction method：Lentivirus

Selection Marker：Puromycin


Parental：HCT 15

Species：Human

Morphology：Epithelioid

Growth properties：Adherent

Culture Medium：RPMI-1640 supplemented with 10 % FBS,4.0ug/mL Puromycin,   100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G186L).

Passage：1:3-1:4

Doubling time：24-36 h

Subculture：Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:4.

Freezing media：RPMI-1640+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).


Chen TR , et al. DLD-1 and HCT-15 cell lines derived separately from colorectal carcinomas have totally different chromosome changes but the same genetic origin. Cancer Genet. Cytogenet. 81: 103-108, 1995. PubMed: 7621404 22861: Trainer DL , et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874 23033: Dexter DL , et al. N,N-dimethylformamide-induced alteration of cell culture characteristics and loss of tumorigenicity in cultured human colon carcinoma cells. Cancer Res. 39: 1020-1025, 1979. PubMed: 427742 32266: Bender CM , et al. Inhibition of DNA methylation by 5-Aza-2'-deoxycytidine suppresses the growth of human tumor cell lines. Cancer Res. 58: 95-101, 1998. PubMed: 9426064 46971: Vermeulen SJ , et al. Did the four human cancer cell lines DLD-1, HCT-15, HCT-8, and HRT-18 originate from one and the same patient?. Cancer Genet. Cytogenet. 107: 76-79, 1998. PubMed: 9809040
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Accelerate research, empower industry