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Luciferase Reporter HEK-293 Cell Line(HEK-293-Luc)
Luciferase Reporter HEK-293 Cell Line(HEK-293-Luc) 
Cat. code: TCH-C190L
Unit size: 1x10^6
Abbreviation: HyCyte HEK-293-Luc
Price:
$1,260.00
General Info
Cell Info
References


Product NameLuciferase Reporter HEK-293 Cell Line

HyCyte cell lineHyCyte HEK-293-Luc

Catalog No.TCH-C190L

Construction method:Lentivirus

Selection Marker:Puromycin


ParentalHEK-293

SpeciesHuman

MorphologyEpithelioid

Growth propertiesAdherent

Culture Medium:Dulbecco's Modified Eagle Medium ,High Glucose (DMEM)    supplemented with 10 % FBS,1.0 ug/mL Puromycin, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G190L)

Passage:1:4-1:8

Doubling time:24-48 h

Subculture:Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:4-1:8.

Freezing media:DMEM+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).


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Da Costa LT, et al. Converting cancer genes into killer genes. Proc. Natl. Acad. Sci. USA 93: 4192-4196, 1996. PubMed: 8633039

Graham FL, et al. Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J. Gen. Virol. 36: 59-72, 1977. PubMed: 886304

Graham FL, et al. Defective transforming capacity of adenovirus type 5 host-range mutants. Virology 86: 10-21, 1978. PubMed: 664220

Harrison T, et al. Host-range mutants of adenovirus type 5 defective for growth in HeLa cells. Virology 77: 319-329, 1977. PubMed: 841862

Bodary SC, McLean JW. The integrin beta 1 subunit associates with the vitronectin receptor alpha v subunit to form a novel vitronectin receptor in a human embryonic kidney cell line. J. Biol. Chem. 265: 5938-5941, 1990. PubMed: 1690718

Goodrum FD, Ornelles DA. The early region 1B 55-kilodalton oncoprotein of adenovirus relieves growth restrictions imposed on viral replication by the cell cycle. J. Virol. 71: 548-561, 1997. PubMed: 8985383"

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