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Luciferase Reporter HeLa Cell Line(HeLa-Luc)
Luciferase Reporter HeLa Cell Line(HeLa-Luc) 
Cat. code: TCH-C193L
Unit size: 1x10^6
Abbreviation: HyCyte HeLa-Luc
Price:
$1,260.00
General Info
Cell Info
References


Product NameLuciferase Reporter HeLa Cell Line

HyCyte cell lineHyCyte HeLa-Luc

Catalog No.TCH-C193L

Construction method:Lentivirus

Selection Marker:Puromycin


ParentalHeLa

SpeciesHuman

MorphologyEpithelioid

Growth propertiesAdherent

Culture Medium:Minimum Essential Medium (MEM) supplemented with 10 % FBS,2.0 ug/mL Puromycin, 100 U/ml Penicilin and 100 µg/mlStreptomycin (HyCyte,TCH-G193L).

Passage:1:2-1:6

Doubling time:48-72 h

Subculture:Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:6.

Freezing media:MEM+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).


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AOAC International Invasiveness by Escherichia coli of mammalian cells, microbiological method. Gaithersburg, MD:AOAC International;AOAC ""Official Methods of Analysis of the AOAC International"" 982.36.

Baldi A, et al. Genomic structure of the human retinoblastoma-related Rb2/p130 gene. Proc. Natl. Acad. Sci. USA 93: 4629-4632, 1996. PubMed: 8643454

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Chen TR. Re-evaluation of HeLa, HeLa S3, and HEp-2 karyotypes. Cytogenet. Cell Genet. 48: 19-24, 1988. PubMed: 3180844

Boshart M, et al. A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer. EMBO J. 3: 1151-1157, 1984. PubMed: 6329740

Schneider-Gadicke A, Schwarz E. Different human cervical carcinoma cell lines show similar transcription patterns of human papillomavirus type 18 early genes. EMBO J. 5: 2285-2292, 1986. PubMed: 3023067

Schwarz E, et al. Structure and transcription of human papillomavirus sequences in cervical carcinoma cells. Nature 314: 111-114, 1985. PubMed: 2983228

Pater MM, Pater A. Human papillomavirus types 16 and 18 sequences in carcinoma cell lines of the cervix. Virology 145: 313-318, 1985. PubMed: 2992153

Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217

Scheffner M, et al. The state of the p53 and retinoblastoma genes in human cervical carcinoma cell lines. Proc. Natl. Acad. Sci. USA 88: 5523-5527, 1991. PubMed: 1648218

Jones HW Jr., et al. George Otto Gey. (1899-1970). The HeLa cell and a reappraisal of its origin. Obstet. Gynecol. 38: 945-949, 1971. PubMed: 4942173"

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