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Luciferase Reporter Hep3B 2.1-7 Cell Line(Hep3B 2.1-7-Luc)
Luciferase Reporter Hep3B 2.1-7 Cell Line(Hep3B 2.1-7-Luc) 
Cat. code： TCH-C195L
Unit size： 1x10^6
Abbreviation： HyCyte Hep3B 2.1-7-Luc
Price：
$1,260.00
General Info
Cell Info
References


Product Name：Luciferase Reporter Hep3B 2.1-7 Cell Line

HyCyte cell line：HyCyte Hep3B 2.1-7-Luc

Catalog No.：TCH-C195L

Construction method：Lentivirus

Selection Marker：Puromycin


Parental：Hep3B 2.1-7

Species：Human

Morphology：Epithelioid

Growth properties：Adherent

Culture Medium：Minimum Essential Medium (MEM) supplemented with 10 % FBS,6.0 ug/mL Puromycin,1%NEAA, 2mM Glutamax, 1mM Sodium Pyruvate,100 U/ml Penicilin and 100 µg/mlStreptomycin (HyCyte,TCH-G195L).

Passage：1:2-1:3

Doubling time：72-96 h

Subculture：Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:3.

Freezing media：MEM+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Knowles BB, et al. Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 209: 497-499, 1980. PubMed: 6248960.

Knowles BB, Aden DP. Human hepatoma derived cell line, process for preparation thereof, and uses therefor. US Patent 4,393,133 dated Jul 12 1983.

Aden DP, et al. Controlled synthesis of HBsAg in a differentiated human liver carcinoma- derived cell line. Nature 282: 615-616, 1979. PubMed: 233137.

Darlington GJ, et al. Growth and hepatospecific gene expression of human hepatoma cells in a defined medium. In Vitro Cell. Dev. Biol. 23: 349-354, 1987. PubMed: 3034851.

Knowles BB, Aden DP. Human hepatoma derived cell line, process for preparation thereof, and uses therefor. US Patent 4,393,133 dated Jul 12 1983.
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