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Luciferase Reporter HT-29 Cell Line(HT-29-Luc)
Luciferase Reporter HT-29 Cell Line(HT-29-Luc) 
Cat. code： TCH-C213L
Unit size： 1x10^6
Abbreviation： HyCyte HT-29-Luc
Price：
$1,260.00
General Info
Cell Info
References


Product Name：Luciferase Reporter HT-29 Cell Line

HyCyte cell line：HyCyte HT-29-Luc

Catalog No.：TCH-C213L

Construction method：Lentivirus

Selection Marker：Puromycin


Parental：HT-29

Species：Human

Morphology：Epithelioid

Growth properties：Adherent

Culture Medium：McCoy's 5A Medium supplemented with 10 % FBS, 2.0ug/mL Puromycin,100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G213L).

Passage：1:3-1:6

Doubling time：48-72 h

Subculture：Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media：McCoy's 5A + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).


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Hanski C, et al. Tumorigenicity, mucin production and AM-3 epitope expression in clones selected from the HT-29 colon carcinoma cell line. Int. J. Cancer 50: 924-929, 1992. PubMed: 1372882

Reiter LS, et al. The role of the urokinase receptor in extracellular matrix degradation by HT29 human colon carcinoma cells. Int. J. Cancer 53: 444-450, 1993. PubMed: 8381394

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Shabahang M, et al. 1,25-Dihydroxyvitamin D3 receptor as a marker of human colon carcinoma cell line differentiation and growth inhibition. Cancer Res. 53: 3712-3718, 1993. PubMed: 8393379"
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