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Luciferase Reporter HuH-7 Cell Line(HuH-7-Luc)
Luciferase Reporter HuH-7 Cell Line(HuH-7-Luc) 
Cat. code： TCH-C217L
Unit size： 1x10^6
Abbreviation： HyCyte HuH-7-Luc
Price：
$1,260.00
General Info
Cell Info
References


Product Name：Luciferase Reporter HuH-7 Cell Line

HyCyte cell line：HyCyte HuH-7-Luc

Catalog No.：TCH-C217L

Construction method：Lentivirus

Selection Marker：Puromycin


Parental：HuH-7

Species：Human

Morphology：Epithelioid

Growth properties：Adherent

Culture Medium： Dulbecco's Modified Eagle Medium ,High Glucose (DMEM)    supplemented with 10 % FBS,4.0 ug/mL Puromycin, 2mM Glutamax, 1mM Sodium Pyruvate,100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G217L）

Passage：1:2-1:3

Doubling time：48-72 h

Subculture：Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:3.

Freezing media：DMEM+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).


Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A.

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Cancer Res. 79:1263-1273(2019)

Kasai F., Hirayama N., Ozawa M., Satoh M., Kohara A.

HuH-7 reference genome profile: complex karyotype composed of massive loss of heterozygosity.

Hum. Cell 31:261-267(2018)

Shi J., Wang X., Lyu L., Jiang H., Zhu H.-J.

Comparison of protein expression between human livers and the hepatic cell lines HepG2, Hep3B, and Huh7 using SWATH and MRM-HR proteomics: Focusing on drug-metabolizing enzymes.

Drug Metab. Pharmacokinet. 33:133-140(2018)
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