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Product Name：Luciferase/GFP labelled human human lymphoma cells

HyCyte cell line：Jeko-1-Luc-GFP

Catalog No：TCH-C224LG

Construction method：PiggyBac

Selection Marker：Puromycin

Parental：Jeko-1

Species：Human

Morphology：Lymphoblastic

Growth properties：Suspension

Culture Medium： RPMI-1640 supplemented with 20 % FBS,0.5 ug/mL Puromycin, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G224LG).

Passage：1:4-1:6

Doubling time： 24-32 h

Subculture：Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:4-1:6.

Freezing media：RPMI-1640+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).