Accelerate research, empower industry

CRISPR/Cas9-mediated gene knockout (KO)CRISPR/Cas9-mediated gene knockin (KI)CRISPR/Cas9-mediated gene point-mutationInducible Gene Over-expression (Tet-On)shRNA Knockdown cell serviceCRISPR-KO libraryCRISPRa libraryCRISPRi libraryCRISPRSeek™ library screening serviceCRISPRSeek™ Library PlasmidCRISPRSeek™ Library VirusCRISPRSeek™ Library Cell PoolCRISPRSeek™ Library ProductsHySigen Vector LibraryVB163VB164VB203VB204VB205VB303VB271VB272VB188VB278VB180VB251VB252Custom Vector Construction ServiceCustom Vector Construction Service-1Lentivirus PackagingshRNA AAVAdeno-Associated Virus (AAV) PackagingOver-expression AAVIn-stock virusesVirus PackagingCustomized Peptide SynthesisHuman cell linesAnimal cell linesCell LinesKO cell linesPoint mutation cell linesKI cell linesFluorescence-tag cell linesStable cell linesOff-the-shelf gene-edited cell linesOne-step freezing medium for stem cellsOne-step freezing medium for PBMCsCell freezing mediaOsteogenic differentiation culture mediumAdipogenic differentiation culture mediumChondrogenic differentiation culture mediumStem cell differentiation mediaStem cell culture mediaKnockout Cell LinesKnockin Cell LinesPoint mutation cell lineInducible Gene ExpressionGenome-Wide Libraries at Unbeatable PricesWho we arePartnersDistributorsTechnical PlatformsNews & eventsPublicationsResourcesPrivacy policySupportContact UsSubmitted successfully
插件代码
Luciferase Reporter Jurkat Cell Line(Jurkat-Luc)
Luciferase Reporter Jurkat Cell Line(Jurkat-Luc) 
Price：
$1,260.00
Cat.No.：
Size：
Quantity：
Order
Product info
Documents
References
Cat.No.： TCH-C225L Size： 1x10^6

Product Name：Luciferase Reporter Jurkat Cell Line

HyCyte cell line：HyCyte Jurkat-Luc

Catalog No.：TCH-C225L

Construction method：Lentivirus

Selection Marker：Puromycin

Parental：Jurkat

Species：Human

Morphology：Lymphoblastic

Growth properties：Suspension

Culture Medium：RPMI-1640 supplemented with 10 % FBS,3.0ug/mL Puromycin,   2mM Glutamax, 1mM Sodium Pyruvate,100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G225L)

Passage：1:3-1:6

Doubling time：24-48 h

Subculture：Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media：RPMI-1640+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).


"Weiss A, et al. The role of T3 surface molecules in the activation of human T cells: a two-stimulus requirement for IL-2 production reflects events occurring at a pre-translational level. J. Immunol. 133: 123-128, 1984. PubMed: 6327821

Gillis S, Watson J. Biochemical and biological characterization of lymphocyte regulatory molecules. V. Identification of an interleukin 2-producing human leukemia T cell line. J. Exp. Med. 152: 1709-1719, 1980. PubMed: 6778951

Berninghausen O, Leippe M. Necrosis versus apoptosis as the mechanism of target cell death induced by Entamoeba histolytica. Infect. Immun. 65: 3615-3621, 1997. PubMed: 9284127

Churchill MJ, et al. The rev-responsive element negatively regulates human immunodeficiency virus type 1 env mRNA expression in primate cells. J. Virol. 70: 5786-5790, 1996. PubMed: 8709194

Kolanus W, et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed: 8706128

Gan W, Rhoads RE. Internal initiation of translation directed by the 5'-untranslated region of the mRNA for eIF4G, a factor involved in the picornavirus-induced switch from cap-dependent to internal initiation. J. Biol. Chem. 271: 623-626, 1996. PubMed: 8557663

Tiffany HL, et al. Enhanced expression of the eosinophil-derived neurotoin ribonuclease (RNS2) gene requires interaction between the promoter and intron. J. Biol. Chem. 271: 12387-12393, 1996. PubMed: 8647842

Chan YJ, et al. Synergistic interactions between overlapping binding sites for the serum response factor and ELK-1 proteins mediate both basal enhancement and phorbol ester responsiveness of primate cytomegalovirus. J. Virol. 70: 8590-8605, 1996. PubMed: 8970984

Kung SH, Medveczky PG. Identification of a herpesvirus saimiri cis-acting DNA fragment that permits stable replication of episomes in transformed T cells. J. Virol. 70: 1738-1744, 1996. PubMed: 8627695

Bloom TJ, Beavo JA. Identification and tissue-specific expression of PDE7 phosphodiesterase splice variants. Proc. Natl. Acad. Sci. USA 93: 14188-14192, 1996. PubMed: 8943082"
Contact us
Title
Name
*
Country/Region
*
How would you like us to contact you?
*
E-mail
*
Institution
Phone
Where did you learn about HySigen?
Verification code
Change Image
*
Submit

Quote/Order

We will contact you with information about this product. Please let us know how to reach you.
Contact Us


Address: 56 Sugar Creek Blvd Suite 375, Sugar Land, TX 77478

Email: info@hysigen.com

Telephone: 628-777-8169 (US)

Accelerate research, empower industry