Accelerate research, empower industry

CRISPR/Cas9-mediated gene knockout (KO)CRISPR/Cas9-mediated gene knockin (KI)CRISPR/Cas9-mediated gene point-mutationInducible Gene Over-expression (Tet-On)shRNA Knockdown cell serviceCRISPR-KO libraryCRISPRa libraryCRISPRi libraryCRISPRSeek™ library screening serviceCRISPRSeek™ Library PlasmidCRISPRSeek™ Library VirusCRISPRSeek™ Library Cell PoolCRISPRSeek™ Library ProductsHySigen Vector LibraryVB163VB164VB203VB204VB205VB303VB271VB272VB188VB278VB180VB251VB252Custom Vector Construction ServiceCustom Vector Construction Service-1Lentivirus PackagingshRNA AAVAdeno-Associated Virus (AAV) PackagingOver-expression AAVIn-stock virusesVirus PackagingCustomized Peptide SynthesisHuman cell linesAnimal cell linesCell LinesKO cell linesPoint mutation cell linesKI cell linesFluorescence-tag cell linesStable cell linesOff-the-shelf gene-edited cell linesOne-step freezing medium for stem cellsOne-step freezing medium for PBMCsCell freezing mediaOsteogenic differentiation culture mediumAdipogenic differentiation culture mediumChondrogenic differentiation culture mediumStem cell differentiation mediaStem cell culture mediaKnockout Cell LinesKnockin Cell LinesPoint mutation cell lineInducible Gene ExpressionGenome-Wide Libraries at Unbeatable PricesWho we arePartnersDistributorsTechnical PlatformsNews & eventsPublicationsResourcesPrivacy policySupportContact UsSubmitted successfully
插件代码
Luciferase Reporter K-562 Cell Line(HyCyte K-562-Luc)
Luciferase Reporter K-562 Cell Line(HyCyte K-562-Luc) 
Price：
$1,260.00
Cat.No.：
Size：
Quantity：
Order
Product info
Documents
References
Cat.No.： TCH-C226L Size： 1x10^6

Product Name：Luciferase Reporter K-562 Cell Line

HyCyte cell line：HyCyte K-562-Luc

Catalog No.：TCH-C226L

Construction method：Lentivirus

Selection Marker：Puromycin

Parental：K-562

Species：Human

Morphology：Lymphoblastoid

Growth properties：Suspension

Culture Medium：Iscove’s Modified Dulbecco Medium(IMDM) supplemented with 10 % FBS, 4.0 ug/mL Puromycin,100 U/ml Penicilin and 100 µg/ml Streptomycin (TCH-G226L).

Passage：1:3-1:6

Doubling time：24-48 h

Subculture：Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media：IMDM+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).


"Koeffler HP, Golde DW. Human myeloid leukemia cell lines: a review. Blood 56: 344-350, 1980. PubMed: 6996765

Ortaldo JR, et al. Specificity of natural cytotoxic reactivity of normal human lymphocytes against a myeloid leukemia cell line. J. Natl. Cancer Inst. 59: 77-82, 1977. PubMed: 69036

Lozzio CB, Lozzio BB. Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome. Blood 45: 321-334, 1975. PubMed: 163658

Lozzio BB, Lozzio CB. Properties and usefulness of the original K-562 human myelogenous leukemia cell line. Leuk. Res. 3: 363-370, 1979. PubMed: 95026

Andersson LC, et al. K562--a human erythroleukemic cell line. Int. J. Cancer 23: 143-147, 1979. PubMed: 367973

Lozzio BB, et al. A multipotential leukemia cell line (K-562) of human origin. Proc. Soc. Exp. Biol. Med. 166: 546-550, 1981. PubMed: 7194480

Dimery IW, et al. Variation amongst K562 cell cultures. Exp. Hematol. 11: 601-610, 1983. PubMed: 6576909

Chan YJ, et al. Two distinct upstream regulatory domains containing multicopy cellular transcription factor binding sites provide basal repression and inducible enhancer characteristics to the immediate-early IES (US3) promoter from human cytomegalovirus. J. Virol. 70: 5312-5328, 1996. PubMed: 8764042

Kolanus W, et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed: 8706128

Gan W, Rhoads RE. Internal initiation of translation directed by the 5'-untranslated region of the mRNA for eIF4G, a factor involved in the picornavirus-induced switch from cap-dependent to internal initiation. J. Biol. Chem. 271: 623-626, 1996. PubMed: 8557663

Tiffany HL, et al. Enhanced expression of the eosinophil-derived neurotoin ribonuclease (RNS2) gene requires interaction between the promoter and intron. J. Biol. Chem. 271: 12387-12393, 1996. PubMed: 8647842"
Contact us
Title
Name
*
Country/Region
*
How would you like us to contact you?
*
E-mail
*
Institution
Phone
Where did you learn about HySigen?
Verification code
Change Image
*
Submit

Quote/Order

We will contact you with information about this product. Please let us know how to reach you.
Contact Us


Address: 56 Sugar Creek Blvd Suite 375, Sugar Land, TX 77478

Email: info@hysigen.com

Telephone: 628-777-8169 (US)

Accelerate research, empower industry