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Luciferase Reporter KYSE-150 Cell Line(KYSE-150-Luc)
Luciferase Reporter KYSE-150 Cell Line(KYSE-150-Luc) 
Cat. code： TCH-C236L
Unit size： 1x10^6
Abbreviation： HyCyte KYSE-150-Luc
Price：
$1,260.00
General Info
Cell Info
References


Product Name：Luciferase Reporter KYSE-150 Cell Line

HyCyte cell line：HyCyte KYSE-150-Luc

Catalog No.：TCH-C236L

Construction method：Lentivirus

Selection Marker：Puromycin


Parental：KYSE-150

Species：Human

Morphology：Epithelioid

Growth properties：Adherent

Culture Medium：RPMI-1640 supplemented with 10 % FBS,2.0 ug/mL Puromycin, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G236L).

Passage：1:2-1:4

Doubling time：24-48 h

Subculture：Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media：RPMI-1640+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).


Kanda, Y., Nishiyama, Y., Shimada, Y., Imamura, M., Nomura, H., Hiai, H., Fukumoto, M. (1994). Analysis of gene amplification and overexpression in human esophageal-carcinoma cell lines. International journal of cancer.Journal international du cancer 58 ( 2 ): 291-297 . Http://www.ncbi.nlm.nih.gov/pubmed/7913084 Shimada, Y., Imamura, M., Wagata, T., Yamaguchi, N., Tobe, T. (1992). Characterization of 21 newly established esophageal cancer cell lines. Cancer 69 ( 2 ): 277-284 . Http://www.ncbi.nlm.nih.gov/pubmed/1728357 Shimada, Y., Imamura, M. (1993). Prognostic significance of cell culture in carcinoma of the oesophagus. The British journal of surgery 80 ( 5 ): 605-607 . Http://www.ncbi.nlm.nih.gov/pubmed/8518899 Tanaka, H., Shibagaki, I., Shimada, Y., Wagata, T., Imamura, M., Ishizaki, K. (1996). Characterization of p53 gene mutations in esophageal squamous cell carcinoma cell lines: increased frequency and different spectrum of mutations from primary tumors. International journal of cancer.Journal international du cancer 65 ( 3 ): 372-376 . Http://www.ncbi.nlm.nih.gov/pubmed/8575860
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