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Luciferase Reporter LNCaP Cell Line(LNCaP-Luc)
Luciferase Reporter LNCaP Cell Line(LNCaP-Luc) 
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Cat.No.: TCH-C238L Size: 1x10^6

Product NameLuciferase Reporter LNCaP Cell Line

HyCyte cell lineHyCyte LNCaP-Luc

Catalog No.TCH-C238L

Construction method:Lentivirus

Selection Marker:Puromycin

ParentalLNCaP

SpeciesHuman

MorphologyEpithelioid

Growth propertiesAdherent

Culture Medium:RPMI-1640 supplemented with 10 % FBS,2.0ug/mL Puromycin,   100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G238L).

Passage:1:2-1:3

Doubling time:72-96 h

Subculture:Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:3.

Freezing media:RPMI-1640+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).


Models for prostate cancer. 37New York: Liss; 1980.

Gibas Z, et al. A high-resolution study of chromosome changes in a human prostatic carcinoma cell line (LNCaP). Cancer Genet. Cytogenet. 11: 399-404, 1984. PubMed: 6584201

Horoszewicz JS, et al. LNCaP model of human prostatic carcinoma. Cancer Res. 43: 1809-1818, 1983. PubMed: 6831420

Hu SX, et al. Development of an adenovirus vector with tetracycline-regulatable human tumor necrosis factor alpha gene expression. Cancer Res. 57: 3339-3343, 1997. PubMed: 9269991

Boffa LC, et al. Invasion of the CAG triplet repeats by a complementary peptide nucleic acid inhibits transcription of the androgen receptor and TATA-binding protein genes and correlates with refolding of an active nucleosome containing a unique AR gene sequence. J. Biol. Chem. 271: 13228-13233, 1996. PubMed: 8662737

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