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Luciferase Reporter MHCC97-H Cell Line( MHCC97-H-Luc)
Luciferase Reporter MHCC97-H Cell Line( MHCC97-H-Luc) 
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Cat.No.： TCH-C258L Size： 1x10^6

Product Name：Luciferase Reporter MHCC97-H Cell Line

HyCyte cell line：HyCyte MHCC97-H-Luc

Catalog No.：TCH-C258L

Construction method：Lentivirus

Selection Marker：Puromycin

Parental：MHCC97-H

Species：Human

Morphology：Epithelioid

Growth properties：Adherent

Culture Medium：Dulbecco's Modified Eagle Medium ,High Glucose (DMEM)    supplemented with 10 % FBS,6.0 ug/mL Puromycin, 2mM Glutamax, 1mM Sodium Pyruvate,100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G258L）

Passage：1:3-1:6

Doubling time：48-72 h

Subculture：Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media：DMEM+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).


1. Dang H., Steinway S.N., Ding W., Rountree C.B.

Induction of tumor initiation is dependent on CD44s in c-Met(+) hepatocellular carcinoma.

BMC Cancer 15:161-161(2015)

2. Tian M.-M., Cheng H., Wang Z.-Q., Su N., Liu Z.-X., Sun C.-Q., Zhen B., Hong X.-C., Xue Y., Xu P.

Phosphoproteomic analysis of the highly-metastatic hepatocellular carcinoma cell line, MHCC97-H.

Int. J. Mol. Sci. 16:4209-4225(2015)

3. Wang K., Lim H.Y., Shi S., Lee J., Deng S., Xie T., Zhu Z., Wang Y., Pocalyko D., Yang W.J., Rejto P.A., Mao M., Park C.-K., Xu J.

Genomic landscape of copy number aberrations enables the identification of oncogenic drivers in hepatocellular carcinoma.

Hepatology 58:706-717(2013)

4. Li Y., Tian B., Yang J., Zhao L., Wu X., Ye S.-L., Liu Y.-K., Tang Z.-Y.

Stepwise metastatic human hepatocellular carcinoma cell model system with multiple metastatic potentials established through consecutive in vivo selection and studies on metastatic characteristics.

J. Cancer Res. Clin. Oncol. 130:460-468(2004)

5. Ding S.-J., Li Y., Shao X.-X., Zhou H., Zeng R., Tang Z.-Y., Xia Q.-C.

Proteome analysis of hepatocellular carcinoma cell strains, MHCC97-H and MHCC97-L, with different metastasis potentials.

Proteomics 4:982-994(2004)
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