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Human non-small cell lung carcinoma cell line(NCI-H1975)
Human non-small cell lung carcinoma cell line(NCI-H1975) 
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Cat.No.： TCH-C276 Size： 1x10^6

Cell Name: Human non-small cell lung adenocarcinoma cell line

Cell Synonyms:NCI-H1975; H1975; H-1975

Catalogue No.: TCH-C276

Species: Human
Tissue Origin: Lung
Disease Characteristics: Lung adenocarcinoma

Morphology: Lymphoblast-like
Growth Properties: Adherent

Culture Medium: RPMI-1640 + 10% FBS +1% P/S
Matching Medium Catalog Number: TCH-G276
Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 36-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection


NCI-H1975 STR profile

Loci

Test Results for Submitted Sample

Sample

Allele1

Allele2

Amelogenin

X


D3S1358

14

15

D5S818

11

12

D2S1338

17


TPOX

8

11

CSF1PO

12


Penta D

12

13

TH01

7


vWA

18


D7S820

8


D21S11

28


Penta E

12

16

D10S1248

13


D8S1179

13

16

D1S1656

14


D18S51

13


D12S391

17


D6S1043

12


D19S433

15

15.2

D16S539

9

12

D13S317

10


FGA

21

24


Manual Simple-Set-of-Stationery-Related-Vector-Line-Icons 1.svg

Published articles on NCI-H1975:

IF=8.7 Yang C, Li H X, Gan H, et al. KRAS4B oncogenic mutants promote non-small cell lung cancer progression via the interaction of deubiquitinase USP25 with RNF31[J]. Developmental cell, 2025.

IF=3.5 He W, Wei M, Huang Y, et al. Integrated Bioinformatics Analysis and Cellular Experimental Validation Identify Lipoprotein Lipase Gene as a Novel Biomarker for Tumorigenesis and Prognosis in Lung Adenocarcinoma[J]. Biology, 2025, 14(5): 566.
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